tcna1275saf Rabbit Anti-Human IgG Heavy Chain Antibody- sodium azide free

IHC testing of human tonsil and IgG antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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$498.00 ORDERING INFORMATION International
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Product Description

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationFACS, IF, IHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesRabbit
Immunogen / Amino acidsHuman Ig Gamma Chain was used as the immunogen for this IgG antibody.
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeRabbit IgG

Specifications

FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RTThe optimal dilution of the IgG antibody for each application should be determined by the researcher.

Misc Information

Storage InstructionStore the IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein A affinity chromatography
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