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Secondary Antibody
Rabbit Anti-Human IgG Heavy Chain Antibody- sodium azide free
tcna1275saf
Rabbit Anti-Human IgG Heavy Chain Antibody- sodium azide free
IHC testing of human tonsil and IgG antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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AVAILABLE SIZES
100ug
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
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Heat mediated
Enzymatic
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Product Description
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
Application
FACS, IF, IHC-P
Species Reactivity
Human. Other species not known.
Host Species
Rabbit
Immunogen / Amino acids
Human Ig Gamma Chain was used as the immunogen for this IgG antibody.
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration
1 mg/ml
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RTThe optimal dilution of the IgG antibody for each application should be determined by the researcher.
Misc Information
Storage Instruction
Store the IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProt
P01857
/
P01859
/
P01860
/
P01861
References
Protein A affinity chromatography
related data
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