tcba13666 Rabbit anti GST-Tag Polyclonal Antibody

Western blot analysis of GST-tagged protein using GST-tag antibody at different dilution. Each lane was loaded with 5 ug protein.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Western blot analysis of over-expressed GST fusion protein using GST-tag antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IF, IP
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human GST-Tag
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:1000 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
PurificationAffinity purification
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