tcba13667 Mouse anti HA-Tag Monoclonal Antibody

Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb. Blue: DAPI for nuclear staining.Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody. A mock served as negative control and over-expressed 293T cell lysate served as positive control.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IF, IP
Host SpeciesMouse
Immunogen / Amino acidsA synthetic peptide of YPYDVPDYA (HA-tag)-KLH
ConjugationUnconjugated
ClonalityMonoclonal
IsotypeIgG

Specifications
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:2000 - 1:5000
IF 1:50 - 1:500
IP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
PurificationAffinity purification
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