tcba13636 β actin Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using β-actin antibody at 1:100000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Western blot analysis of extracts of HeLa cells, using β-actin antibody at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody at dilution of 1:100 (20x lens).Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody at dilution of 1:100 (20x lens).Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody at dilution of 1:100 (20x lens).Immunofluorescence analysis of HeLa cells using ACTB antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Order Now

AVAILABLE SIZES

 $124.96 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Information
ApplicationWB, IHC
Research AreaEpigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation, Epigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation,
Species ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human β-actin
ConjugationUnconjugated
ClonalityMonoclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:50000 - 1:200000
IHC 1:200 - 1:2000

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesACTB; BRWS1; PS1TP5BP1; Beta-Actin;Actin
SwissProtP60709
Gene ID60 (human);
Calculated Molecular Weight41kDa
PurificationAffinity purification
NotesLoading control antibody
ProtocolPut 5ul into 45ul Buffer (with 50% glycerol), dilute 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot
Cellular LocationCytoplasm,cytoskeleton,
Get valuable resources and offers directly to your email.
COMPANY About Taiclone Shipping Terms Taiclone Guarantee
RESOURCES Life Science Glossary
PRODUCTS Primary AntibodySecondary AntibodyProtein & PeptidesBioChemicalsAssay & Elisa KitsLoading ControlOther ReagentsSerumsCell Line
SUPPORT Contact Us Email Us Terms of Service Privacy Policy
Copyright 2016-2023 Taiclone Biotech Corp. All Rights Reserved.