tcba8023 GEMIN2 Polyclonal Antibody

Western blot analysis of extracts of Jurkat cells, using GEMIN2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat liver using GEMIN2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using GEMIN2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human lymphonodus using GEMIN2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using GEMIN2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human vermiform appendix using GEMIN2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using GEMIN2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using GEMIN2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using GEMIN2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GEMIN2 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of A549 cells using GEMIN2 antibody. Blue: DAPI for nuclear staining.
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Product Description
This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

Information
ApplicationWB, IHC, IF
Research AreaNeuroscience,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesSIP1;SIP1-delta
SwissProtO14893
Gene ID8487 (human);
Calculated Molecular Weight4kDa/28kDa/29kDa/31kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,gem,
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