Product Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Information
ApplicationWB, IHC, IF, IP, ChIP, ChIPseq
Research AreaCancer, MAPK pathway, MAPK/p38 pathway, MAPK/ERK pathway, Epigenetics,
Species ReactivityHuman, Mouse, Rat, Other (Wide Range)
Host SpeciesRabbit
IsotypeIgG
Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesH3.4;H3/g;H3FT;H3t
Calculated Molecular Weight15kDa
PurificationAffinity purification
Cellular LocationChromosome,Nucleus,