tcba7455 DiMethyl-Histone H3-K27 pAb

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody at 1:1000 dilution.Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody. Blue: DAPI for nuclear staining.Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Information

ApplicationWB, IHC, IF, IP, ChIP, ChIPseq
Research AreaCancer, MAPK pathway, MAPK/p38 pathway, MAPK/ERK pathway, Epigenetics,
Species ReactivityHuman, Mouse, Rat, Other (Wide Range)
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesH3.4;H3/g;H3FT;H3t
SwissProtQ16695
Gene ID8290 (human);
Calculated Molecular Weight15kDa
PurificationAffinity purification
Cellular LocationChromosome,Nucleus,
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