tcba3468 CYCS Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using CYCS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.Immunohistochemistry of paraffin-embedded rat testis using CYCS antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat ovary using CYCS antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using CYCS antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using CYCS antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using CYCS antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using CYCS antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using CYCS antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using CYCS antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using CYCS antibody at dilution of 1:150 (40x lens).Immunofluorescence analysis of HeLa cells using CYCS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Information

ApplicationWB, IHC, IF
Research AreaCancer, Apoptosis, Neuroscience, Cardiovascular, Cell Biology, Metabolism, Neurodegeneration,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCYC;HCS;THC4
SwissProtP99999
Gene ID54205 (human);
Calculated Molecular Weight11kDa
PurificationAffinity purification
Cellular LocationMitochondrion intermembrane space,
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