tcba13056 Phospho-PRKAA1-T183 / PRKAA2-T172 pAb

Western blot analysis of extracts of C2C12 cells, using Phospho-PRKAA1-T183/PRKAA2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-PRKAA1-T183-PRKAA2-T172 antibody at dilution of 1:100 (40x lens).Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-PRKAA1-T183/PRKAA2-T172 pAb. Western blot was performed from the immunoprecipitate using Phospho-PRKAA1-T183/PRKAA2-T172 pAb at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IP, IHC
Research AreaCancer, Neuroscience, Cardiovascular, Cell Biology, Metabolism, Lipid Metabolism pathway,
Species ReactivityMouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IP 1:50 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
SwissProtQ13131 / P54646
Gene ID5562/5563 (human);
Calculated Molecular Weight64kDa/65kDa
PurificationAffinity purification
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