tcba11931 METTL3 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using METTL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 1s.Immunohistochemistry of paraffin-embedded rat brain using METTL3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using METTL3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using METTL3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using METTL3 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of C6 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody. Western blot was performed from the immunoprecipitate using METTL3 antibody at a dilition of 1:1000.
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Product Description
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Information
ApplicationWB, IHC, IF, IP
Research Area,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
SwissProtQ86U44
Gene ID56339 (human);
Calculated Molecular Weight25kDa/64kDa
PurificationAffinity purification
Cellular LocationNucleus speckle,
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