tcba11717 IL33 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using IL33 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat brain using IL33 antibody at dilution of 1:100 (40x lens).
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Product Description
The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.

Information
ApplicationWB, IHC
Research AreaCardiovascular, Immunology, Innate Immunity,
Species ReactivityHuman, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:200 - 1:2000
IHC 1:50 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesC9orf26;DVS27;IL1F11;NF-HEV;NFEHEV
SwissProtO95760
Gene ID90865 (human);
Calculated Molecular Weight16kDa/25kDa/26kDa/30kDa
PurificationAffinity purification
Cellular LocationChromosome,Cytoplasmic vesicle,Nucleus,Secreted,secretory vesicle,
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