tcba11505 ADAR Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using ADAR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 20s.Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using ADAR antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using ADAR antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Information

ApplicationWB, IHC, IF
Research AreaRNA Binding Protein(RBP),
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesADAR1;AGS6;DRADA;DSH;DSRAD;G1P1;IFI-4;IFI4;K88DSRBP;P136
SwissProtP55265
Gene ID103 (human);
Calculated Molecular Weight103kDa/131kDa/133kDa/136kDa/140kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,Nucleus,nucleolus,
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