tcba10634 NASP Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using NASP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using NASP Antibody at dilution of 1:100 (40x lens).
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Product Description

This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.

Information

ApplicationWB, IHC
Research AreaCancer, Cell cycle, Cell Biology,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesFLB7527;HMDRA1;PRO1999
SwissProtP49321
Gene ID4678 (human);
Calculated Molecular Weight48kDa/78kDa/85kDa/86kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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