tcba9641 TP53BP1 Polyclonal Antibody

Western blot analysis of extracts of A-431 cells, using TP53BP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat kidney using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using TP53BP1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using TP53BP1 antibody. Blue: DAPI for nuclear staining.Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using TP53BP1 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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$140.00 ORDERING INFORMATION International
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Product Description

No product descriptions available. Please refer to the product information & specifications for details.

Information

ApplicationWB, IHC, IF
Research AreaCancer, p53 pathway, DNA Damage/Repair,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative Names53BP1;p202;p53BP1;TDRD30;TP53
SwissProtQ12888
Gene ID7158 (human);
Calculated Molecular Weight213kDa/214kDa
PurificationAffinity purification
Cellular LocationChromosome,Nucleus,centromere,kinetochore,
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