tcba9619 ACTR2 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using ACTR2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human liver cancer using ACTR2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using ACTR2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using ACTR2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using ACTR2 antibody. Blue: DAPI for nuclear staining.
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Product Description
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.

Information
ApplicationWB, IHC, IF
Research AreaCancer,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesARP2
SwissProtP61160
Gene ID10097 (human);
Calculated Molecular Weight44kDa/45kDa
PurificationAffinity purification
Cellular LocationCell projection,Cytoplasm,cytoskeleton,
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