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Primary Antibody
ZEB2 Polyclonal Antibody
tcba9592
ZEB2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ZEB2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ZEB2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using ZEB2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ZEB2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ZEB2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using ZEB2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ZEB2 antibody at dilution of 1:100 (40x lens).
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
Information
Application
WB, IHC
Research Area
Cancer, Stem cells, Neuroscience, Epigenetics,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
HSPC082;SIP-1;SIP1;SMADIP1;ZFHX1B
SwissProt
O60315
Gene ID
9839
(human);
Calculated Molecular Weight
133kDa/136kDa
Purification
Affinity purification
Cellular Location
Nucleus,
related data
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