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Primary Antibody
FLI1 Polyclonal Antibody
tcba9531
FLI1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FLI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody. Western blot was performed from the immunoprecipitate using FLI1 antibody at a dilition of 1:1000.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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CELLS
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Product Description
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
Information
Application
WB, IHC, IP
Research Area
Cancer, Cell cycle, Apoptosis, Cell Biology, Metabolism, Epigenetics,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
BDPLT21;EWSR2;SIC-1;EWSR2;SIC-1
SwissProt
Q01543
Gene ID
2313
(human);
Calculated Molecular Weight
29kDa/43kDa/47kDa/50kDa
Purification
Affinity purification
Cellular Location
Nucleus,
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