tcba9410 SNRPE Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SNRPE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.Immunohistochemistry of paraffin-embedded human kidney using SNRPE Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPE Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using SNRPE Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of U2OS cells using SNRPE antibody. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesHYPT11;Sm-E;SME;snRNP-E
SwissProtP62304
Gene ID6635 (human);
Calculated Molecular Weight10kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,cytosol,
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