tcba9360 RPS6KA3 Polyclonal Antibody

Western blot analysis of extracts of mouse lung, using RPS6KA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat lung using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody. Blue: DAPI for nuclear staining.
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Product Description
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

Information
ApplicationWB, IHC, IF
Research AreaCancer, mTOR pathway, MAPK pathway, MAPK/ERK pathway,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCLS;HU-3;ISPK-1;MAPKAPK1B;MRX19;p90-RSK2;pp90RSK2;RSK;RSK2;S6K-alpha3
SwissProtP51812
Gene ID6197 (human);
Calculated Molecular Weight83kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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