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Primary Antibody
NLK Polyclonal Antibody
tcba8114
NLK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NLK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using NLK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using NLK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using NLK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NLK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NLK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NLK antibody at dilution of 1:100 (40x lens).
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$
140.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
Other
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.
Information
Application
WB, IHC
Research Area
Cancer, Stem cells, Cell cycle, Wnt pathway, Developmental Biology, Cell Biology,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
SwissProt
Q9UBE8
Gene ID
51701
(human);
Calculated Molecular Weight
58kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,Nucleus,
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