tcba8044 NFKB2 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using NFKB2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat liver using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver injury using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human vermiform appendix using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat lung using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterus using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse skeletal muscle using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using NFKB2 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using NFKB2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.

Information

ApplicationWB, IHC, IF
Research AreaCancer, Apoptosis, Cell Biology, NF-κB pathway, Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCVID10;H2TF1;LYT-10;LYT10;NF-kB2;p100;p49/p100;p52
SwissProtQ00653
Gene ID4791 (human);
Calculated Molecular Weight47kDa/96kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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