tcba7666 KRT5 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using KRT5 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using KRT5 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using KRT5 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using KRT5 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using KRT5 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using KRT5 antibody.
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Product Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCK5;DDD;DDD1;EBS2;K5;KRT5A
SwissProtP13647
Gene ID3852 (human);
Calculated Molecular Weight62kDa
PurificationAffinity purification
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