tcba7177 KPNA4 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using KPNA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat brain using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon using KPNA4 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophagus using KPNA4 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using KPNA4 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using KPNA4 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colonic muscle using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using KPNA4 Antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of U2OS cells using KPNA4 antibody. Blue: DAPI for nuclear staining.
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Product Description
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesIPOA3;QIP1;SRP3
SwissProtO00629
Gene ID3840 (human);
Calculated Molecular Weight57kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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