tcba6241 ELAVL1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using ELAVL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat testis using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophagus using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using ELAVL1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat cancer using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using ELAVL1 Antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of MCF-7 cells using ELAVL1 antibody. Blue: DAPI for nuclear staining.
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Product Description

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Information

ApplicationWB, IHC, IF
Research AreaRNA Binding Protein(RBP),
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesELAV1;Hua;HUR;MelG
SwissProtQ15717
Gene ID1994 (human);
Calculated Molecular Weight36kDa/38kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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