tcba4780 CD36 Polyclonal Antibody

Western blot analysis of extracts of THP-1 cells, using CD36 antibody at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) at 37℃ for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 15s.Western blot analysis of extracts of various cell lines, using CD36 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 15s.Customer Review: Western Blot analysis of NRK52E Cells with CD36 Polyclonal Antibody at Dilution 1:200Customer Submit Review: Immunohistochemistry of paraffin-embedded B6 Mice liver section using CD36 antibody at dilution of 1:200 (40x lens).Western blot analysis of extracts of various cell lines, using BRD2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunofluorescence analysis of THP-1 cells using CD36 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunohistochemistry of paraffin-embedded rat liver using CD36 antibody at dilution of 1:100 (40x lens).
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Product Description

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.

Information

ApplicationWB, IF
Research AreaCancer, Stem cells, Cardiovascular, Immunology,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant fusion protein containing a sequence corresponding to amino acids 301-400 of human CD36 (NP_001120916.1).
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCD36; BDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3; CD36 molecule
SwissProtP16671
Gene ID948 (human);
Calculated Molecular Weight32kDa/46kDa/48kDa/53kDa
Observed Molecular Weight70-110kDa
SequenceASPVENPDNYCFCTEKIISKNCTSYGVLDISKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFTLQFAKRLQVNLLVKPSEKIQ
PurificationAffinity purification
Positive ControlTHP-1, Jurkat, U-937, HeLa, 293T, Mouse kidney
Cellular LocationCell membrane,Multi-pass membrane protein,
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