tcba657 YAP1 Polyclonal Antibody

KOVALIDATED
Western blot analysis of extracts of various cell lines, using YAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat heart using YAP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using YAP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using YAP1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Information
ApplicationWB, IHC, IF, IP
Research AreaCancer, Hippo pathway, Developmental Biology, Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCOB1;YAP;YAP2;YAP65;YKI
SwissProtP46937
Gene ID10413 (human);
Calculated Molecular Weight36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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