tcip3049 Recombinant Musoke marburgvirus Glycoprotein minus the Transmembrane Region (MMARV rGPΔTM) His-tagged

(Panel A) SDS-PAGE demonstrating 1 g and 5 g (lane 1, 2 respectively) of RAVV rGPΔmuc protein under denaturing and reducing conditions. MW denotes Novex Sharp pre-stained protein standard. (Panel B and C) Western blot detection of RAVV rGPΔmuc at 50 ng, 100 ng and 500 ng (lanes 1-3) under denaturing and reducing conditions. (B) RAVV rGPΔmuc was detected using Taiclone's anti-MARV GP2 polyclonal antibody (cat# 0303-007) at 0.5 ug/mL and anti-rabbit IgG-HRP conjugate, followed by TMB membrane substrate. (C) RAVV rGPΔmuc was detected using IBT’s anti-MARV GP1 monoclonal antibody at 0.5 ug/mL and anti-mouse IgG-HRP conjugate, followed by TMB membrane substrate. Plate was coated with RAVV rGPΔmuc starting at 800 ng/well, serially diluted in DPBS. The washed plate was detected using one dilution of a positive control serum, followed with anti-IgG-HRP conjugate and TMB microwell substrate. OD 650 is reported. Background of RAVV rGPΔmuc without positive control serum was 0.062 OD 650.
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Product Description
Mature, recombinant, His-tagged Musoke marburgvirus Glycoprotein minus the transmembrane domain (MMARV rGPΔTM) is supplied as a purified protein. MMARV rGP TM is produced in mammalian cells and is purified by FPLC.

Information
ApplicationELISA, WB
Research AreaVirology

Specifications
ConcentrationSupplied in (supplemented with glycerol, arginine and glutamic acid) at a concentration of 0.331 mg/mL.
Recommended DilutionSDS-PAGE: The theoretical molecular weight of the
protein is ~71 kDa without glycosylation. Because of the highly glycosylated nature of this protein
migration in an SDS-PAGE gel is slowed resulting in broad
diffuse bands representing differing glycosylation forms Western Blot: Quality control testing demonstrates detection of MMARV rGPΔTM under reduced conditions down to 50 ng when using Taiclone's polyclonal antibody (cat# tcia147) at 0.5 g/mL. Quality control testing demonstrates strong detection of GP null and GP2 under reduced conditions

Misc Information
Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceRecombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.
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