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Protein & Peptides
EcoCRM197 Diptheria Toxin Mutant Vaccine Carrier Protein
tcip3045
EcoCRM197 Diptheria Toxin Mutant Vaccine Carrier Protein
The theoretical molecular weight of the protein is ~68 kDa including the His-tag, without glycosylation. (A) SDS-PAGE and stain demonstrating 5 µg and 1 µg (lanes 1, 2 respectively) of EBOV rGPΔTM His-tag protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein marker. (B) Western blot detection of EBOV rGPΔTM at 500 ng, 100 ng and 50 ng (lanes 1-3, respectively) using rabbit anti-EBOV GP ®polyclonal antibody (catalog# tcia135) at 0.5µg/mL and anti-rabbit IgG-HRP conjugate, followed by TMB membrane substrate.
Plate was coated with EBOV rGPΔTM starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMB substrate. OD 650 is reported. Background of EBOV rGPΔTM coated plate positive control serum was 0.073 OD650.
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1mg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Product Description
EcoCRM™ is CRM 197 produced as a soluble intracellular protein in E. coli. CRM 197 is genetically detoxified diphtheria toxin. Specifically, it has a single amino acid substitution (glycine52 to glutamic acid), which causes the ADPribosyltransferase activity to be lost. CRM 197 is widely used as the carrier protein for conjugate vaccines. EcoCRM™ is expressed as a recombinant protein in Escherichia coli. ;
The theoretical molecular weight of the protein is 58.4 kDa.;
Supplied by: Fina Biosolutions LLC
Information
Application
ELISA, WB
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
1mg/ml in 1X PBS, pH 7.6, 10% glycerol.; Endotoxin: 0.16 EU/µg
Purity / Grade
N/A
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
CRM 197 is widely used as the carrier protein for conjugate vaccines. Vaccines for Haemophilus influenzae b, meningococcal disease and Streptococcus pneumoniae use CRM 197;Unliketoxoids, CRM 197 has its full complement of lysines available for conjugation. It is also thought to cause less interference in multivalent vaccines. EcoCRM™ is stable in PBS.
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