tcip3037 Recombinant S. aureus Gamma Hemolysin B (Hlg B) (tag-free)

(A) SDS-PAGE and stain demonstrating 1 µg and 5 µg (lanes 1 and 2, respectively) of Hlg C protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers.; (B) Western blot detection of Hlg C at 100 ng, 50 ng, and 25 ng (lanes 1-3). Hlg C was detected using IBT’s polyclonal antibody at 500 ng/mL and anti-rabbit IgG-horseradish peroxidase (HRP) conjugate, followed by TMB substrate.
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Product Description
Purified, tag-free Staphylococcus aureus Gamma Hemolysin B (Hlg B) expressed in E. coli. ;

The theoretical molecular weight of the protein is 35.4 kDa.

Information
ApplicationELISA, WB, Cytotoxicity
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied in PBS at a concentration of 1.596 mg/mL
Recommended DilutionCytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with Hlg A or Hlg C.
Purity / GradeColumn chromatography (FPLC)

Misc Information
Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceThis protein may be used in functional toxicity assays in combination with Hlg A, Hlg C, or as a control protein in ELISA assays or Western blotting.
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