Products
PRIMARY ANTIBODY
SECONDARY ANTIBODY
PROTEIN & PEPTIDES
BIOCHEMICALS
ASSAY & ELISA KITS
LOADING CONTROL
OTHER REAGENTS
SERUMS
CELL LINE
Contact Us
About Us
Login
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Products
Contact Us
About Us
Shopping Cart
Loading....
Login Account
Sign In
Register
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Home
Protein & Peptides
Recombinant S. aureus Gamma Hemolysin A (Hlg A) (tag free)
tcip3036
Recombinant S. aureus Gamma Hemolysin A (Hlg A) (tag free)
(A) SDS-PAGE and stain demonstrating 1 µg and 5 µg (lanes 1 and 2, respectively) of Hlg B protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers.; (B) Western blot detection of Hlg B at 200 ng, 100 ng, 50 ng, 25 ng (lanes 1-4). Hlg B was detected using Taiclone's polyclonal antibody (cat# tcia159) at 50 ng/mL and anti-rabbit IgG-horseradish peroxidase (HRP) conjugate, followed by TMB substrate.
Order Now
AVAILABLE SIZES
100µg
$
0.00
Add to Cart
ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
Download PDF Datasheet
Technical Inquries
Submit Review
Submit Review
Please choose your application, enter the information requested below. * Marked fields are mandatory.
Full Name
Email
Contact Number
Application
Please Select..
Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
Select One
Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
Select One
Heat mediated
Enzymatic
Other
None
Positive Control
Negative Control
Dilution *
1:
Incubation Time *
Select Image *
Choose a file...
Rating *
Remarks *
Submit Review
Product Description
Purified, Staphylococcus aureus recombinant Gamma Hemolysin A (Hlg A). The rHlg A (tag free) is expressed in E. coli and purified by FPLC. ;
The theoretical molecular weight of the protein is 34,956 Daltons.
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS at a concentration of 2.065 mg/mL; Protein demonstrates a molecular weight of approximately 35 kDa. ; Endotoxin: 3.332 Endotoxin Units/mg
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution
internal QC demonstrates detection of 50 ng of rHlg A protein using anti-PVL Luk
polyclonal antibody (cat# 04-0009) in Western blotting.
Cytotoxicity assay: Cytotoxicity can be detected in human
neutrophils when used in combination with rHlg B in a
concentration range of 4-200 nM.
Purity / Grade
Column chromatography (FPLC)
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
This protein may be used in functional toxicity assays in combination with rHlg B, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
related data
Get valuable resources and offers directly to your email.
REGISTER NOW