tcip3036 Recombinant S. aureus Gamma Hemolysin A (Hlg A) (tag free)

Data sheet

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Product Description

Purified, Staphylococcus aureus recombinant Gamma Hemolysin A (Hlg A). The rHlg A (tag free) is expressed in E. coli and purified by FPLC. ;

The theoretical molecular weight of the protein is 34,956 Daltons.

Information

ApplicationELISA, WB, Cytotoxicity
Research AreaVirology

Specifications

FormFrozen Liquid
ConcentrationSupplied in PBS at a concentration of 2.065 mg/mL; Protein demonstrates a molecular weight of approximately 35 kDa. ; Endotoxin: 3.332 Endotoxin Units/mg
Recommended DilutionELISA: Assay-dependent dilution.

WB: Assay-dependent dilution
internal QC demonstrates detection of 50 ng of rHlg A protein using anti-PVL Luk
polyclonal antibody (cat# 04-0009) in Western blotting.

Cytotoxicity assay: Cytotoxicity can be detected in human
neutrophils when used in combination with rHlg B in a
concentration range of 4-200 nM.
Purity / GradeColumn chromatography (FPLC)

Misc Information

Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceThis protein may be used in functional toxicity assays in combination with rHlg B, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
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