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Product Description
Purified, Staphylococcus aureus recombinant Gamma Hemolysin A (Hlg A). The rHlg A (tag free) is expressed in E. coli and purified by FPLC. ;
The theoretical molecular weight of the protein is 34,956 Daltons.
Information
ApplicationELISA, WB, Cytotoxicity
Research AreaVirology
Specifications
FormFrozen Liquid
ConcentrationSupplied in PBS at a concentration of 2.065 mg/mL;
Protein demonstrates a molecular weight of approximately 35 kDa. ;
Endotoxin: 3.332 Endotoxin Units/mg
WB: Assay-dependent dilution internal QC demonstrates detection of 50 ng of rHlg A protein using anti-PVL Luk polyclonal antibody (cat# 04-0009) in Western blotting.
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rHlg B in a concentration range of 4-200 nM.
Purity / GradeColumn chromatography (FPLC)
Misc Information
Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceThis protein may be used in functional toxicity assays in combination with rHlg B, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
related data
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