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Protein & Peptides
Recombinant Toxic Shock Syndrome Toxin-1 (TSST-1)
tcip3035
Recombinant Toxic Shock Syndrome Toxin-1 (TSST-1)
(A) SDS-PAGE of rHlg A: 1 ug (lane 1) and 5 ug (lane 2). ; (B) Western blot detection of rHlg A at 200 ng, 100 ng and 50 ng (lanes 1-3), usingTaiclone's anti-PVL Luk S polyclonal antibody (Cat# 04-0009) at 0.5 µg/mL and an anti-rabbit IgG-HRP conjugate followed by substrate.
Toxin Functionality: Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of rHlg A and rHlg B at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690nm. EC 50 values were found to be 7.12 nM for the current lot 1504002 (blue circles) and 6.67 nM for the previous lot 1311002 (red circles).
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100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Recombinant TSST-1 expressed in E.coli and purified to >90% using chromatographic methods.
Information
Application
ELISA, WB, Cytotoxicity, Stimulation
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS + 10% Glycerol at a concentration of 1.3 mg/mL. ; The theoretical molecular weight of the TSST-1 is 22,204 Daltons.
Recommended Dilution
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution
internal QC demonstrates strong detection of 1 ng of TSST-1
PBMC Stimulation assay: Stimulation of PBMCs to produce Interferon γ by TSST-1 can be achieved with 0.1 ng/mL.
Purity / Grade
Column chromatography (FPLC)
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
TSST-1 is a pyrogenic toxin superantigen secreted by Staphylococcus aureus. ; TSST-1 is a powerful activator of T-lymphocytes resulting in substantial cytokine release that can cause severe pathological consequences ; Related Products: Anti- S. aureus Superantigens Polyclonal Antibody Catalog # tcia157; Anti- S. aureus LukS-PV Monoclonal Antibody Catalog # tcia126 ; Anti- S. aureus Alpha-Hemolysin Polyclonal Antibody Catalog # tcia175
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