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Protein & Peptides
Staphylococcus aureus Phenol Soluble Modulin-Alpha 3 (PSM-α3)
tcip3034
Staphylococcus aureus Phenol Soluble Modulin-Alpha 3 (PSM-α3)
SDS-PAGE and staining results for TSST-1 (A) at 11 µg and 5 µg respectively. Western blot detection (B) of 100 and 500 ng of TSST-1 respectively using IBT’s polyclonal rabbit anti-Staphylococcus aureus Superantigen antibody at 0.5 µg/mL followed by an anti-rabbit alkaline phosphatase conjugate and visualized with AP substrate.
Interferon γ was detected from PBMCs by ELISA following 48 hour incubation of PBMCs with varying amounts of TSST-1.
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AVAILABLE SIZES
100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Product Description
Purified Staphylococcus aureus phenol soluble modulin (PSM-α3) peptide.
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in phosphate buffered saline at a concentration of 1.00 mg/mL
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Not recommended for this peptide.
Cytotoxicity assay: The peptide is active in functional lysis assays with horse red blood cells and human neutrophils (Data not shown). Lytic activity towards human or rabbit RBC for this product has not been tested. The peptide can be also used in serology assay as coating antigen to detect antibodies to PSM.
Purity / Grade
Column chromatography (FPLC)
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
S. aureus secretes four short (~20 amino acids) α-type phenol soluble modulins of which the PSM- α3 plays the most prominent role in S. aureus virulence. ; PSM toxin is a major virulence factor of S. aureus and primarily causes cytolysis in red and white blood cells.
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