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Protein & Peptides
Recombinant Staphylococcus aureus Alpha Hemolysin (Hla) (tag free)
tcip3032
Recombinant Staphylococcus aureus Alpha Hemolysin (Hla) (tag free)
Lysis of horse red blood cells by S. aureus δ-Toxin. Red blood cell lysis was measured by the OD of the supernatants at 416nm after 45 min incubation at 37°C with δ-Toxin. ; Red circles represents the current lot 1508005 and green circles represents the previous lot 1410001.
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AVAILABLE SIZES
100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Staphylococcus aureus alpha hemolysin (Hla), also known as alpha toxin (α-toxin), is expressed in E. coli as a recombinant protein without TAG. ;The theoretical molecular weight of the protein is approximately 33 kDa.
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in phosphate buffered saline at a concentration of 0.520 mg/mL. ; Endotoxin: 30.213 Endotoxin units/mg
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution
internal QC demonstrates detection of Hla protein using Taiclone's mouse anti-Alpha Toxin (6C12) monoclonal antibody (cat# tcia123) in Western blotting.
Cytotoxicity assay: The protein is active in functional lysis assays with rabbit red blood cells.
The protein can be also used in serology assay as coating antigen to detect antibodies against Hla.
Purity / Grade
Column chromatography (FPLC)
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
Hla is produced by S. aureus and is a major cytotoxic agent. It is expressed as a monomer that binds to the membrane of susceptible cells. ; Subunits then oligomerize to form heptameric rings with a central pore through which cellular contents leak. The mode of action of S. aureus Hla is likely by osmotic lysis.
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