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Protein & Peptides
Recombinant SUDV Soluble GP (sGP)
tcip3031
Recombinant SUDV Soluble GP (sGP)
(A) SDS-PAGE of Hla: 1 ug (lane 1) and 5 ug (lane 2). ; (B) Western blot detection of Hla at 5 ng,10 ng and 50 ng (lanes 1-3), using Taiclone's mouse anti-Alpha Toxin (6C12) monoclonal antibody (cat# tcia123) at 1.0 µg/mL and an anti-mouse IgG-HRP conjugate followed by TMB membrane substrate.
Lysis of rabbit red blood cells by S. aureus Hla. Red blood cell lysis was determined by absorbance at OD 416 nm after 30 min incubation at 37°C with Hla. EC 50 values were found to be 0.0322 µg/mL for lot 1805007 (blue circles) and 0.0301 µg/mL for previous lot 1601002 (green circles).
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AVAILABLE SIZES
100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
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Product Description
Mature, recombinant Sudan Virus Soluble Glycoprotein (SUDV sGP) containing a c-terminal HIS tag, is supplied as purified protein. SUDV sGP is produced in mammalian cells and is purified by FPLC.;The theoretical molecular weight of the protein is ~47 kDa. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in a broad, diffuse band representing differing glycosylation forms.
Information
Application
ELISA, WB
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 0.686 mg/mL.
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
Relevance
Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.
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