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Protein & Peptides
Recombinant S. aureus LukS-PV (tag-free)
tcip3019
Recombinant S. aureus LukS-PV (tag-free)
(A) SDS-PAGE of LukF-PV: 1 µg (lane 1) and 5 µg (lane 2) Protein demonstrates a molecular weight of approximately 37 kDa.; (B) Western blot detection of LukF-PV at 100 ng, usingTaiclone's antiLukF-PV polyclonal antibody (Cat: tcia158) at 0.5 µg/mL and an anti-rabbit IgG-HRP conjugate, followed by TMB substrate.
Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of LukF-PV and LukS-PV at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubating for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. EC 50 values were found to be 0.907 nM.
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AVAILABLE SIZES
100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Product Description
Purified, tag-free Staphylococcus aureus PantonValentine Leukocidin (PVL) S subunit (LukS-PV) ;
The theoretical molecular weight of the protein is 32,465 Dalton
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS at a concentration of 1.709 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution
internal QC demonstrates detection of 100 ng of LukS-PV protein when detected with antiPVL LukS polyclonal antibody (cat# tcia174) in Western blotting.
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukF-PV in a concentration range of 1-100 nM.
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
This protein may be used in functional PVL toxicityassays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus
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