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Protein & Peptides
S. aureus Recombinant LukE (tag free)
tcip3018
S. aureus Recombinant LukE (tag free)
A) SDS-PAGE of LukS-PV: 1 µg (lane 1) and 5 µg (lane 2). (B) Western blot detection of LukS-PV at 100 ng, using Taiclone's antiLukS-PV polyclonal antibody (Cat# tcia174) at 0.5 µg/mL and an anti-rabbit IgG-HRP conjugate, followed by TMB substrate.
Toxin Functionality: Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of LukS-PV and LukF-PV at equimolar concentration for 3 hours at 37°C with 5%CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubating for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. EC50 values were found to be 2.92 nM for the LukSPV tag-free lot 1503008 (red circles) and 4.38 nM for the LukS-PV his-tag lot 1108003 (black circles).
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AVAILABLE SIZES
100µg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Product Description
Purified, Staphylococcus aureus recombinant leukocidin-E (rLukE). ;
The rLukE (tag free) is expressed in E. coli and purified by FPLC. ;
The theoretical molecular weight of the protein is 34,819 Daltons.
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS at a concentration of 2.417 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa.
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution
internal QC demonstrates detection of 100 ng of rLukE protein using anti-LukS-PV polyclonal antibody (cat# 04-0009) by Western blot analysis.
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rLukD in a concentration range of 25-800 nM.
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
This protein may be used in functional toxicity assays in combination with rLukD, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
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