tcip3013 Recombinant S. aureus LukA-LukB (tag-free)

(Panel A) SDS-PAGE demonstrating 5 g and 1 g (lane 1, 2 respectively) of EBOV rGPΔmuc protein under denaturing and reducing conditions. MW denotes Novex Sharp pre-stained protein standard. (Panel B) Western blot detection of EBOV rGPΔmuc at 500 ng, 100 ng and 50 ng (lanes 1-3) under denaturing and reducing conditions. EBOV rGPΔmuc was detected using an anti EBOV 1® rGPΔmuc polyclonal antibody at 2.0 g/mL and anti-rabbit IgGHRP conjugate, followed by TMB membrane substrate. The theoretical molecular weight of the protein is 56 kDa. Plate was coated with EBOV rGPΔmuc starting at 800 ng/well, serially diluted in DPBS. The washed plate was detected using one dilution of a positive control serum, followed with anti-IgG-HRP conjugate and TMB microwell substrate. OD 650 is reported. Background of EBOV rGPΔmuc without positive control serum was 0.071 OD 650.
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Product Description
Purified, tag-free recombinant Staphylococcus aureus LukA-LukB heterodimer. ;

The theoretical molecular weight of LukA is 37,082 Daltons and the molecular weight of LukB is 35,573 Daltons.

Information
ApplicationWB
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied at a concentration of 1.524 mg/mL in sodium phosphate buffer containing sodium chloride and 5% glycerol.
Recommended DilutionAssay-dependent dilutions.
Purity / GradeColumn chromatography

Misc Information
Storage Instruction2-3 weeks at -20°C, -80°C long term
RelevanceThis protein may be used in functional HL-60 toxicity assay, or as a control protein when detecting LukA-LukB in strains of S. aureus.
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