tcip3009 Recombinant AMARV GPDTM

(A) SDS-PAGE of LASV Z: 1 µg (lane 1) and 5 µg (lane 2). ; (B) Western blot detection of LASV Z at 100 ng, 50 ng and 25 ng (lanes 1-3), using IBT’s rabbit anti-LASV Z polyclonal antibody (cat # 0307-002) at 0.1 µg/mL and an anti-rabbit IgG-HRP conjugate followed by substrate.Plate was coated with LASV Z starting at 25 ng/well,serially diluted two-fold in DPBS. ; Washed plate was detected using 10 µg/mL of Rabbit anti-LASV Z pAb followed with anti-rabbit IgG HRP conjugate and TMB substrate. OD 650 is reported.
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Product Description
Mature, recombinant, His-tagged Angola marburgvirus Glycoprotein minus the transmembrane domain

(MARV-Angola rGPΔTM) is supplied as purified protein. MARVAngola rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC.

Information
ApplicationELISA, WB
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied in PBS (supplemented withglycerol, arginine and glutamic acid) at a concentration of 0.592mg/mL.
Recommended DilutionWestern Blot: Quality control testing demonstrates strong detection of GP null and GP2 under reduced conditions.
Purity / GradeColumn chromatography

Misc Information
Storage Instruction2-3 weeks at -20°C, -80°C long term
RelevanceRecombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.
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