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Protein & Peptides
Recombinant SUDV GPDTM
tcip3006
Recombinant SUDV GPDTM
(A) SDS-PAGE and stain demonstrating 1 µg and 5 µg (lanes 1, 2 respectively) of SUDV rGPΔTM His-tag protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers. (B) Western blot detection of SUDV rGPΔTM at 200 ng, 100 ng and 50 ng (lanes 1-3). SUDV rGPΔTM was detected using Taiclone's anti-SUDV GP monoclonal antibody ® (2H5) (catalog# tcia118) at 0.5 ug/mL and anti-mouse IgG-HRP conjugate, followed by TMB membrane substrate.
Plate was coated with SUDV rGPΔTM starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMB substrate. OD 650 is reported. Background of SUDV rGPΔTM coated plate without positive control serum was 0.088 OD 650 .
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AVAILABLE SIZES
100µg
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Mature, recombinant, His-tagged Sudan
virus Glycoprotein minus the transmembrane domain
(SUDV rGPΔTM) is supplied as purified protein. SUDV rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC.
Information
Application
ELISA, WB
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 1.059 mg/mL
Recommended Dilution
Western Blot: Quality control testing demonstrates strong detection of GP null and GP1 under reduced conditions.
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
Recombinant glycoprotein provides a means as a control protein for immunoassays and a tool to enhance research.
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