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Protein & Peptides
Recombinant EBOV VP24
tcip3005
Recombinant EBOV VP24
The theoretical molecular weight of the protein is ~25 kDa. (A) SDS-PAGE and stain demonstrating 1 ug and 5 ug (lanes 1-2) of EBOV VP24 protein under denaturing and reducing conditions. MW denotes Novex Sharp pre-stained protein markers. (B) Anti-EBOV VP24 antibody (tcia140) at 100 ng/mL detected 100 ng of EBOV rVP24 (lane 1) by Western Blot Analysis. Western Blots were developed using an anti-rabbit IgG-HRP conjugate and TMB Membrane substrate. MW denotes Novex ® Sharp pre-stained protein markers.
rVP24 protein was diluted to 3 µg/mL in PBS for plate coating. Anti-EBOV VP40 antibody was serially diluted semi-log from 10 µg/mL and incubated on the coated plates. Washed plates were detected with anti-rabbit IgG-HRP conjugate and TMB substrate. OD650 is reported above.
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AVAILABLE SIZES
100µg
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0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Product Description
Recombinant, HIS-tagged, purified Ebola virus (EBOV) matrix protein (VP24) is expressed in E. coli and the recombinant protein purified using FPLC.
Information
Application
ELISA, WB
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in Tris buffered saline, at a concentration of 2.279 mg/mL
Recommended Dilution
Western Blot: Assay-dependent dilution
ELISA: Assay-dependent dilution
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
store aliquots at -80°C to avoid multiple freeze/thaw cycles.
Relevance
Recombinant EBOV VP24 matrix protein provides a means as a control protein for immunoassays and a tool to enhance Filovirus research.
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