tcip3003 Recombinant Ebola virus Glycoprotein minus theTransmembrane Region (EBOV rGPΔTM)

Data sheet

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Product Description

Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM) is supplied as purified protein. EBOV rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC;

The theoretical molecular weight of the protein is ~68 kDa including the His-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDSPAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.

Information

ApplicationELISA, WB
Research AreaVirology

Specifications

FormFrozen Liquid
Concentrationsupplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 1.591 mg/mL
Recommended DilutionWestern Blot: Quality control testing demonstrates strong detection of GP null and GP1 under reduced conditions.
Purity / GradeColumn chromatography

Misc Information

Storage Instruction2-3 weeks at -20°C, -80°C long term
RelevanceRecombinant glycoprotein provides a means as a control protein for immunoassays and a tool to enhance research.
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