tcia122 Mouse anti-Staphylococcal Alpha Hemolysin Toxin Monoclonal Antibody (8B7)

Western blot was detected with anti-alpha hemolysin mAb 8B7 at 1 µg/mL and visualized using an anti-mouse IgG-HRP conjugate and TMB membrane substrate. MW denotes Novex Sharp prestained protein markers. Alpha-toxin at 100 ng (lane 1) is visualized as a protein band at ~33 kDa. Protein band at ~33 kDa was also detected in the culture supernatant of USA300 (lane 2), but not in the negative control USA300 ΔHla strain (lane 3). Various Staphylococcal toxins were coated to plates at a concentration of 1 µg/mL in PBS. ;Anti-alpha hemolysin mAb 8B7 was serially diluted semi-log from 2 µg/mL and incubated on the toxin coated plates. ;Washed plates were detected with antimouse IgG-HRP conjugate and TMB substrate. OD 650 is reported above.
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Product Description
Mouse monoclonal antibody (mAb) reactive to full length Staphylococcus aureus alpha-hemolysin (alpha-toxin). ;The antibody detects the antigen produced by S. aureus strains in Western Blotting and ELISA and neutralizes the hemolytic activity of the toxin.

Information
ApplicationELISA, WB, Neutralization
Research AreaVirology
Cross reactivityThis antibody does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, this antibody interacts with Protein A in S. aureus culture supernatant via the Fc region.
Toxin neutralization: Using a rabbit RBC lysis assay, EC 50 of this antibody for neutralization of 0.3 ug/mL of alpha-toxin was determined to be 0.676 ug/mL. Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of this antibody for neutralization of 0.3 ug/mL of alpha-toxin was determined to be 0.676 ug/mL.
Host SpeciesMouse
Immunogen / Amino acidsPolypeptide sequence of Staphylococcus alphahemolysin targeting the N terminus of the mature toxin.
ConjugationUnconjugated
Clonalitymonoclonal
Clonesmonoclonal
IsotypeIgG

Specifications
FormFrozen Liquid,Protein A/G Purified
ConcentrationSupplied in PBS at a concentration of 1.68 mg/mL.
Recommended DilutionOptimal use dilution should be determined for each individual application.

Misc Information
Storage Instruction 2-3 weeks +4°C, -20°C long term. Avoid multiple freeze thaws.
Relevancehe antibody can be used for detection and neutralization of alpha-toxin produced by virulent strains of S.aureus.
PurificationProtein A/G
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