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Primary Antibody
Biotinylated Anti-Mouse IgG3 rabbit monoclonal antibody [RM218]
tcra314b
Biotinylated Anti-Mouse IgG3 rabbit monoclonal antibody [RM218]
ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3κ and IgG3λ; No crossreactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 wasused as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG3. The plate was coatedwith different amounts of mouse IgG3. A serialdilution of RM218 was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbit IgGas the secondary antibody.
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AVAILABLE SIZES
50ug
$
329.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Biotinylated Rabbit monoclonal to Mouse IgG3;This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Information
Application
WB, IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG3
Conjugation
Biotin
Clonality
Monoclonal
Clones
RM218
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005 ug/mL-0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P03987
Gene ID
380795
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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