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Primary Antibody
Anti-His-Tag rabbit monoclonal antibody [RM146]
tcra193
Anti-His-Tag rabbit monoclonal antibody [RM146]
Western blot of 293T cells: (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y Using RevMAb Clone RM146 at 0.2 ug/mL.
Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by RevMAb clone RM146, was blotted with RM146. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM146.
Immunohistochemistry staining of naïve HepG2 cells(Negative control) using anti-His-Tag antibody, RM146.
Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, RM146.
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AVAILABLE SIZES
100ug
$
369.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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CELLS
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Product Description
Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag);This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Information
Application
WB, IP, ICC, FC , IHC
Species Reactivity
All Species
Host Species
Rabbit
Immunogen / Amino acids
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Conjugation
Unconjugated
Clonality
Monoclonal
Clones
RM146
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
Western Blot: 0.1 ug/mL – 1 ug/mL
Immunoprecipitation: 0.5 ug/mL – 2 ug/mL
Immunocytochemistry 0.5 ug/mL – 2 ug/mL
Flow Cytometry: 0.5 ug/mL – 2 ug/mL
Immunohistochemistry
0.1 ug/mL-1 ug/mL.
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
Gene ID
N/A
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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