tcra113 Anti-Human IgG4 rabbit monoclonal antibody [RM120]

Detection of human IgG4 in monkey serum.Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgGas the secondary antibody.Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG4 rabbit monoclonal antibody (Clone RM120).ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody RM120 at a 1:50,000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
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Product Description
Rabbit monoclonal to Human IgG4;This antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Information
ApplicationWB, ICC, IHC, FC, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of Human IgG4
ConjugationUnconjugated
ClonalityMonoclonal
ClonesRM120
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration1 mg/mL
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 1-10ug/ml
Immunohistochemistry: 1-10ug/ml (1)

Misc Information
Storage Instructionstore at -20°C ; avoid repeated thawing/freezing
SwissProtP01861
Gene ID3503
PurificationProtein A affinity purified from an animal origin-free culture supernatant
NotesSold under RevMab BioSciences Labelled.
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