Product Description
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Information
ApplicationPep-ELISA, IF, WB
Research Areaprecursor
phosphoprotein
acetylprotein
Species ReactivityHuman, Mouse, Rat, Dog, Pig, Cow
Host SpeciesGoat
Immunogen / Amino acidsC-NEVAKLFNIHED
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3
and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1
Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesAIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; AIF; CMTX4; COWCK; COXPD6; PDCD8; apoptosis-inducing factor 1, mitochondrial; programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor
Reference Sequence No.NP_004199.1; NP_665811.1; NP_665812.1; NP_001124318.1
Calculated Molecular Weight66.9; 66.3; 35.6; 28.4; 35.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
NotesThis antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Positive Controltcva12622p