Product Description
This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Information
ApplicationPep-ELISA, WB
Research Areacysteine protease
apoptosis
diabetes
Alzheimer's disease
Huntington disease
preproprotein
Species ReactivityHuman, Mouse, Rat, Dog
Host SpeciesGoat
Immunogen / Amino acidsC-RDVSKEDHSKRS
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1
NP_001271338.1). An additional band of 65kDa was also consistently observed in MOLT4 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesCASP3; caspase 3; caspase 3, apoptosis-related cysteine protease; HGNC:1504; caspase 3, apoptosis-related cysteine peptidase; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; cysteine protease CPP32; procaspase3
Reference Sequence No.NP_004337.2; NP_116786.1
Calculated Molecular Weight31.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
NotesThis antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Positive Controltcva07286p