tcva06088 Goat anti-PTBP1 / PTB Antibody

EB06088 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.EB06088 (0.1µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.EB06088 (0.01µg/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.EB06088 (0.03µg/ml) staining of lysates of cell line NIH3T3 (35µg protein in RIPA buffer). Detected by chemiluminescence.EB06088 (0.1-0.3µg/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Product Description
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).

Information
ApplicationPep-ELISA, WB, IHC
Research AreaRNA-binding
Viral transmission/ resistance
infectious
disease
immunology
splicing
acetylprotein
Species ReactivityHuman, Mouse, Rat, Dog, Pig, Cow
Host SpeciesGoat
Immunogen / Amino acidsDGIVPDIAVGTKR-C
ConjugationUnconjugated

Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 55kDa band observed in Human
Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa
HepG2
Jurkat
HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.01-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.
Peptide ELISA: antibody detection limit dilution 1:32000.

Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesPTBP1; PTB; polypyrimidine tract binding protein 1; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; HNRNPI; RNA binding protein; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); HNRNP-I; MGC10830; MGC8461; RNA-binding protein; heterogeneous nuclear ribonucleoprotein I
Gene ID29497
Reference Sequence No.NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1
Calculated Molecular Weight59.6, 59.0, 57.2; 21.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
DS Gene Ontology Termspoly-pyrimidine tract binding; heterogeneous nuclear ribonucleoprotein; RNA binding; mRNA processing; mRNA splicing; nucleolus; nucleoplasm
NotesThis antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Positive Controltcva06088p
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