Product Description
This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Information
ApplicationPep-ELISA, WB, IHC
Research Areaadaptor protein
inflammation
apoptosis
cancer
disease
immunology
CARD
apoptosis
Species ReactivityHuman
Host SpeciesGoat
Immunogen / Amino acidsC-RESQSYLVEDLERS
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx. 24kDa band observed in lysates of cell line U937 (calculated MW of 21.6kDa according to NP_037390.2). Recommended for use at 1-3µg/ml.
Peptide ELISA: antibody detection limit dilution 1:32000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesPYCARD; ASC; TMS1; PYD and CARD domain containing; CARD5; MGC10332; TMS; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; TMS-1
Reference Sequence No.NP_037390.2; NP_660183.1
Calculated Molecular Weight21.6; 20.0
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
DS Gene Ontology Termscaspase activation; caspase activator activity; cytoplasm; induction of apoptosis; negative regulation of cell cycle; protein binding; regulation of apoptosis; signal transduction;
NotesThis antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Positive Controltcva05291p