Product Description
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Information
ApplicationPep-ELISA, WB, IHC
Research AreaSH2/SH3 adaptor protein
embryogenesis
cancer
disease
T-cell regulation
immunology
development
Species ReactivityHuman, Mouse, Rat, Dog, Cow
Host SpeciesGoat
Immunogen / Amino acidsC-PRNYVTPVNRNV
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 26kDa band observed in lysates of cell line MOLT4
of Human Thymus and of Human
Mouse
Rat
Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15µg/ml.
Peptide ELISA: antibody detection limit dilution 1:32000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesGRB2; growth factor receptor-bound protein 2; ASH; ASH protein; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; HT027; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3; NCKAP2; OTTHUMP00000166096; OTTHUMP00000166097; OTTHUMP00000166098; abundant SRC homology
Reference Sequence No.NP_002077.1; NP_987102.1
Calculated Molecular Weight25.2; 20.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
DS Gene Ontology TermsSH3/SH2 adaptor protein activity; EGF receptor signaling pathway; RAS protein signal transduction; intracellular signaling cascade; cell-cell signaling
NotesThis antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Positive Controltcva05284p